Composite

Part:BBa_K4007008:Design

Designed by: Jonatan Joel Aliaga Rodriguez   Group: iGEM21_Bolivia   (2021-10-06)


Intracellular arsR density control system (high density)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this part was artificially synthetized and was required to have as few repetitive sequences as possible. ArsR binding sites, RBS and terminators sequences were modified for meeting this requirement.


Source

Based on the constructs generated by Wang. et al [1] but with several modifications

References

[1] Wang, B., Barahona, M., & Buck, M. (2015). Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities. Nucleic Acids Research, 43(3), 1955-1964. https://doi.org/10.1093/nar/gku1388